In-vivo Electroporation

Through out my third rotation in CSHL, I made several constructs first, then tried in-vivo electroporation and finally successed. I did molecular myself, but this complicate experiment is done after watching the old technician doing this several times. He showed me this website, which I just came up and reminded me of writing something.
http://www.m.chiba-u.ac.jp/class/dev/in_vivo_electroporation.html

Weirdly, he can't get the GFP expressed in the brain using this methods. We just thought there're some problems: 1. CMV promoter is not good for electroporation. 2. Our techniques are not mature. He tried twice as many rats as me (I don't have time that much because of having classes and TA in stony brook). But you know what, at the last week of my rotation, the last mother rat give births, I did found that I got expression on the day before I leave!

Finally there is no regret in that rotation. I wrote a protocol and emailed the results altogether to the professor. I know he like me. Actually he feels very sorry of not being able to take me.