Sunday, July 20, 2014

How to change adobe text box property

To change property of the text box (border, fill color), select the text box: press CTRL+E 
To change property of the text  in side the text box (font, color), double click, select the text: press CTRL+E 

Friday, July 18, 2014

E. coli genotypes

http://openwetware.org/wiki/E._coli_genotypes#DH5.CE.B1

DH5α

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ–
  • An Hoffman-Berling 1100 strain derivative (Meselson68)
  • Promega also lists phoA
  • nalidixic acid resistant
  • References:
    • FOCUS (1986) 8:2, 9.
    • Hanahan, D. (1985) in DNA Cloning: A Practical Approach (Glover, D.M., ed.), Vol. 1, p. 109, IRL Press, McLean, Virginia.
    • Grant, S.G.N. et al. (1990) Proc. Natl. Acad. Sci. USA 87: 4645-4649 PMID 2162051.
    • Meselson M. and Yuan R. (1968) Nature 217:1110 PMID 4868368.


XL1-Blue 

endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)
  • nalidixic acid resistant
  • tetracycline resistant (carried on the F plasmid)