Read more carefully

I was kind of complaining on my own that the other person give me a clone without telling me enough information. I have to went to the website and double-check the sequences she gave me. I do see a little difference from the database of the sequence, the stop codon. But it doesn't matter much so I don't need to worry about it.

But then I have to go on to design the cut site to pop this insert out and transfer it to another vector. The two cutting site she left on the VNTI molecule, on purpose, which is suggesting me to use those two, are definitely not good choices. I found a PsiI cutting site right at both ends of the insert on the vector. So this should be perfect.

I went to Barb and ask her to order this enzyme. I can't borrow it from other lab, it seems to be rarely used by researchers. We could not find it on Roche, but finally on NE. Barb ask me what's this for. I explained to her, it's actully she taking care of this clone to some part. Barb said there is actually a SfiI on it, if she remember correctly.

I went back to check. I know the insert I put here is a little bit out of the region of start and stop codon. I went to the website and marked the feature sequences. Then I found it's said there! SfiI site!

If I had read carefully, I would not need to waste so much time looking for the best cutting sites and bother people around. I hate this.

A lesson for myself: Be patient and read carefully!

Listening to Seminars

I like to go to seminars and meetings. To me seminars and key-note talks are more worthwhile to go because from those I could hear a full story, while the 20'-30' doesn't really benefit you much in an overview of that specific field, rather than browsing through the result of the speaker's work.

I make notes whatever talks I went to, like when I go to audit a class. David even asked me once if it (making notes) did anything at all, you would forget it anyway if you don't go back. Actually it's very true.

Today I am thinking I should trace up a little bit whatever talks I went to, I mean if they are somehow interesting or let me learn something. I am making a Wiki myself, specific to my interest.

Just make a note for myself to remind me doing that, since I am such a forgetful person. This is what a graduate student should do.

My night dreams

I make a lot of dreams in the night. It's like my dreams in the night are even more colorful and interesting than my life in the day.

You know what I dreamed of the night before last night?

In the dream, I was very familiar with Barack Obama. I was chatting with him like a friend. Then it comes to the moment that someone (He was well-dressed and I talked to the person too before knowing he is actually the one to decide the president.) announce the next president of America. It's Obama! I was very happy and went to congratulate to him. Shaking hands with him, I said: You should tell NIH to increase the funding to researches!

And then I switched to other dreams.

Oh my god, how can I come to a dream like this? I'm not caring much about the campaign. Except for that I can't avoid to hear those stuff on TV when it comes to a news channel.