Read more carefully

I was kind of complaining on my own that the other person give me a clone without telling me enough information. I have to went to the website and double-check the sequences she gave me. I do see a little difference from the database of the sequence, the stop codon. But it doesn't matter much so I don't need to worry about it.

But then I have to go on to design the cut site to pop this insert out and transfer it to another vector. The two cutting site she left on the VNTI molecule, on purpose, which is suggesting me to use those two, are definitely not good choices. I found a PsiI cutting site right at both ends of the insert on the vector. So this should be perfect.

I went to Barb and ask her to order this enzyme. I can't borrow it from other lab, it seems to be rarely used by researchers. We could not find it on Roche, but finally on NE. Barb ask me what's this for. I explained to her, it's actully she taking care of this clone to some part. Barb said there is actually a SfiI on it, if she remember correctly.

I went back to check. I know the insert I put here is a little bit out of the region of start and stop codon. I went to the website and marked the feature sequences. Then I found it's said there! SfiI site!

If I had read carefully, I would not need to waste so much time looking for the best cutting sites and bother people around. I hate this.

A lesson for myself: Be patient and read carefully!